Research Technologist

Requisition #: 313352

Range: CC

Level: 2

Salary: $13.46 - $18.50 an hour

Status: Casual / On Call

School: School of Medicine

Location: School of Medicine Campus

Location City: Baltimore

Location State: MD

Resume Required for Application: Yes

Area of Interest: Research / Lab

Contact: Human Resources 410-955-2990

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General Description

Department: Oncology (Viral Oncology)
Work Schedule/Hours: Monday-Friday/Varies/ Flexible as needed

The general nature or purpose of the job: Under the general supervision of a faculty investigator, is responsible for maintaining mouse colonies. Work requires basic knowledge of mouse genetics, breeding and techniques gained through previous animal training. Work entails setting up breedings of knockout and transgenic mice and genotyping of these mice. Strong organizational skills are required to maintain records of multiple lines of knockout and transgenic mice. Good oral and written communication skills are required.

Duties and Responsibilities:

  • The research focus of the laboratory is on signaling pathways that regulate innate immunity and viral-induced leukemogenesis. Current projects involve use of knockout and transgenic mice to evaluate the role of specific genes in innate immunity and leukemia/lymphoma induction by the HTLV-1 Tax protein.
  • Manage mouse colonies consisting of three distinct conditional knockout mouse lines (Tax1bp1fl/fl, Rnf11fl/fl, Nemofl/fl) crossed with specific Cre transgenic mice (Lck-Cre or CMV-Cre) to delete genes in a tissue-specific manner. Tax1bp1fl/fl, and Nemofl/fl mice will be crossed with Tax transgenic mice to determine the roles of TAX1BP1 and NEMO in Tax-mediated leukemogenesis. Conventional knockout mice (Zfand6–/– and IL-17RB–/–) will also be maintained.
  • Carry out established mouse breeding schemes, wean and tag pups and genotype mice by PCR using tail DNA.
  • Maintain accurate and current mouse census (dates of birth and death) and breeding records using spreadsheets.
  • Submit deceased mice for necropsy.

    Physical Requirements: • Work will involve handling mice and clipping tails for genotyping. • Finger dexterity is required to open and manipulate PCR tubes.

    Equipment, Machine, or Tool Requirements: • Electrophoresis equipment, centrifuges and PCR. • Operates lab computers (Mac) for data entry. • Proficiency in MS Excel or Animal Colony Management software is preferred.

  • Qualifications

    Bachelor’s degree in biology, chemistry, or related field required. Some related post-undergraduate work experience preferred.

    Licensure, Certification, Registration:
    • On-line IACUC training and possibly live animal training may be required.
    • Enrollment in the Animal Exposure Surveillance Program (AESP) may be required.

    NOTE: The successful candidate(s) for this position will be subject to a pre-employment background check.

    If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the School of Medicine HR Divisional Office at 410-955-2990. For TTY users, call via Maryland Relay or dial 711.

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