Job Req ID:  113088

Research Specialist II

The Johns Hopkins University is seeking a Research Specialist II who will perform laboratory procedures, data processing, and laboratory maintenance following common or established protocols and procedures.

The Single Cell & Transcriptomics Core serves the Johns Hopkins Community as well as the world-wide scientific community. The Core provides cost effective Single Cell and Next Generation sequencing analysis enabling more researchers to access advanced transcriptomics and genomics services. The Core is a fast paced and growing lab that requires additional technicians to aid in sample processing and tracking, lab organization, primary data analysis.

Specific Duties & Responsibilities

  • Under the general supervision of a Core Director or Laboratory Supervisor, the Specialist is responsible for performing quality control analysis on client and Core generated RNA, DNA and library samples and returning data to clients.
  • Prepare libraries for and load, 10x Chromium, Mission Bio Tapestri, Next Generation sequencers or any newly acquired instrument.
  • Perform RNA and DNA extractions and advanced molecular biology sample processing.
  • Analyze raw sequencing data within Illumina BaseSpace software.
  • Analyze raw sequencing data for 10x methods, cell or tissue based.
  • Trains new hires on SOPs, operations, and maintenance of equipment in the core.
  • Perform general lab maintenance, including unpacking orders, signing in projects, and updating sample tracking software.
  • Perform routine maintenance on all instruments, such as cleaning/washing, calibration/system check, software updates, and power cycles.
  • Consults industry tech support to replace kits whenever something fails unexplainably and to troubleshoot what may have caused the problem and how to avoid it in the future.
  • Collaborate with clients/researchers to understand their desired project requirements and deadlines and to provide technical guidance on sample preparation for sequencing.
  • Explain any relevant methods to clients so that they can better understand the technology.
  • Communicate sequencing results and basic data analysis to researchers in a clear and concise manner.
  • Maintain and update sample submission records.
  • Help develop and implement standard operating procedures and best practices for the core facility.
  • Oversee multiple projects simultaneously, ensuring that deadlines are met, and quality standards are maintained.
  • Establish a collaborative learning environment within the lab, promoting continuous skill development and knowledge sharing.
  • Manage inventory, monitors supply levels, orders equipment, and supplies in accordance with established policies and procedures, and initiate the process for equipment repair when needed.
  • Teach/train students, and new technicians in the lab. Guide, direct, and facilitate problem solving for entry-level lab members on day-to-day problems.
  • Understand and use software packages used by SCTC for data analysis.
  • Adhere to all SCTC Core requirements.
  • Perform miscellaneous related duties as assigned.

Minimum Qualifications
  • Bachelor's Degree in Biology, Chemistry or related field.
  • Two years laboratory experience performing relevant techniques.
  • Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

Preferred Qualifications
  • Chromium system.
  • Hands-on experience with 10x.



Classified Title: Research Specialist II 
Role/Level/Range: ACRP/03/MB  
Starting Salary Range: $40,900 - $71,600 Annually ($60,000 targeted; Commensurate with experience) 
Employee group: Full Time 
Schedule: M-F 8:30-5:00 
Exempt Status: Exempt 
Location: School of Medicine Campus 
Department name: ​​​​​​​SOM Bas Sci Inst Service Centers  
Personnel area: School of Medicine 



Total Rewards
The referenced salary range is based on Johns Hopkins University’s good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here:

Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.

Equal Opportunity Employer
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

EEO is the Law:

Accommodation Information
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit

Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. The COVID-19 vaccine does not apply to positions located in the State of Florida. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit and all other JHU applicants should visit

The following additional provisions may apply, depending upon campus. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

School of Medicine - East Baltimore Campus