Research Specialist II
The Whitaker Lab within the Department of Anesthesiology & Critical Care Medicine is seeking a full-time Research Specialist II to join a multidisciplinary group exploring anesthesia, blood pressure, and organ perfusion in both a rat model and a pediatric swine model. The overall goal of this research is to determine the vascular determinants of anesthesia-induced hypotension and to identify organ perfusion-dependent definitions of hypotension in the setting of general anesthesia. Our group uses cutting edge, clinically relevant in vivo and in vitro techniques to evaluate the complex interaction of anesthetic agents, vascular physiology, blood pressure, and organ perfusion to establish best practices for intraoperative management in vulnerable patients. This research is part of an NIH NIGMS funded R35 program aimed at studying the above in animals at the extremes of age. The lab is also interested in perinatal stroke, and a series of experiments are being performed in a piglet model to better understand its pathogenesis and treatment options. The successful applicant will be involved in both research programs.
Specific Duties & Responsibilities
- Requirements include but are not limited to (per PI’s discretion).
Personal Qualifications
- Excellent communication skills and ability to work within a multidisciplinary team.
- Cooperative team spirit.
- Ability to make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction; considers resources and lab priorities when making decisions.
Animal Care/Experimentation
- Anesthesia, endotracheal intubation and surgery/microsurgery, including vascular cannulation, craniotomy, middle cerebral artery occlusion.
- Intraoperative physiologic monitoring and arterial blood gas management.
- Administration of intravenous and intraperitoneal medications.
- Postoperative care.
- Animal feeding & administration of medications.
- Cage clean-up and sanitation.
- Neurobehavioral testing.
Research Support
- Preparation of brain fixatives and buffers.
- Performance of animal perfusions & precise dissection of brain and other organs.
- Preparation of sterile surgical packets.
- Histologic paraffin processing to cut brain specimens.
- Histologic staining.
- Performance of routine essays, such as ELISA and western blotting.
- Data entry, analysis, and figure preparation (preferably in GraphPad Prism).
Administrative Responsibilities
- Maintenance of animal and laboratory records.
- Assist with ACUC protocol preparation and maintenance.
- Maintenance of project-specific documentation (e.g., safety documentation, etc).
- Maintenance of lab inventory, ordering supplies, general lab organization.
Teaching
- Teach investigators, residents, postdocs, and other staff to perform surgical and technical procedures.
- Train personnel in routine animal care, daily maintenance of animals.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field.
- Two years laboratory experience performing relevant techniques.
- Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Certified veterinary technician preferred.
- Four years related laboratory work experience preferred.
- Knowledge of current regulations, policies and principles for the care and use of laboratory animals.
- Knowledge and experience with basic surgical and microsurgical techniques and procedures.
- Ability to handle, restrain and determine the sex of laboratory animals without causing injury or harm to animal, self, and others.
- Ability to understand and follow detailed oral and written instructions.
- Ability to maintain records, read instructions, labels, etc.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $41,300 - $72,300 Annually ($56,250 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: Mon - Fri
Exempt Status: Exempt
Location: School of Medicine Campus
Department name: SOM Ane Pediatric Anesthesiology
Personnel area: School of Medicine