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Job Req ID:  50236

Research Specialist

General Summary/Purpose


Proficient research laboratory specialist with a sophisticated understanding of specific, advanced techniques and a solid foundation in the underlying science, gained from professional experience. Works independently and manages daily research projects.


The Research Specialist participates in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. Capable of independent judgment in the selection, development, performance and evaluation of research methods; operates and maintains appropriate equipment.


Specific Duties & Responsibilities


Performs cell culture and processes biofluids and tissues from human donors and animal models. Performs fast protein liquid chromatography (FPLC)-based size separation to obtain proteins and protein complexes, confirming identity of selected isolated proteins. Isolates RNA, performs quality control, and prepares RNA sequencing libraries using methods including advanced, ligation-free preparations. Separation and characterization of extracellular vesicles. Assists with analysis and graphical representation of RNA-seq data. Troubleshoots and optimizes techniques based on scientific knowledge and research experience. Liases with bioinformatics collaborators.

Follows established procedures or protocols and utilizes aseptic and RNase-free technique to perform laboratory tests and/or experiments which include: conventional and real-time PCR, electrophoretic separations (agarose and acrylamide gels), Western blot, ELISA, cloning and/or subcloning, extracellular vesicle isolation and characterization, electron microscopy, nanoparticle tracking analysis, resistive pulse sensing, flow cytometry, and other molecular and cellular biology techniques.


Learn and train in additional procedures in order to expand skills, foster individual growth, and directly contribute to and facilitate laboratory success.


Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.


Participates in the organization and operation of the laboratory, including oversight of undergraduate student researchers. Performs general lab maintenance duties and maintains excellent written records and electronic databases.


Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.


Minimum Qualifications


Bachelor's degree, or equivalent, in biology, chemistry or related field plus 1 year of laboratory experience performing relevant techniques.


Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula:


* JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job. *


Special Knowledge, Skills, and Abilities


Work produced is subject to precise measures of quantity and quality
Near vision to see objects clearly within 20 inches
Sharp focus to adjust vision when doing close work that changes in distance from eyes
Full spectrum vision to identify and distinguish color
Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard
Handling by seizing, holding, grasping, turning or otherwise working with the hand or hands, but without finger dexterity
Sitting in a normal seated position for extended periods of time
Occasionally lifting, carrying objects weighing 10 lbs. or less
Occasionally pushing, pulling objects weighing 30 lbs. or less
Ability to move about



Classified Title: Research Specialist 
Working Title: Research Specialist  ​​​​​
Role/Level/Range: ACRP/03/MA 
Starting Salary Range: $16.13 - $22.16; Commensurate with Experience
Employee group: Full Time 
Schedule: Monday-Friday, 8:30 am - 5:00 pm, Up to 37.5 hrs/ week 
Exempt Status: Non-Exempt  
Location: 04-MD:School of Medicine Campus 
Department name: 10002778-SOM Comp Med Retrovirus Bio Laboratory 
Personnel area: School of Medicine


The successful candidate(s) for this position will be subject to a pre-employment background check.


If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at For TTY users, call via Maryland Relay or dial 711.


The following additional provisions may apply depending on which campus you will work.  Your recruiter will advise accordingly.


During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.


The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.


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School of Medicine - East Baltimore Campus