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Job Req ID:  16249

Research Specialist II


The Department of Anesthesiology, Research is seeking a Research Specialist II to work under the general supervision of a faculty investigator or laboratory supervisor, the successful candidate will be responsible for performing experiments on animal or human fluids and/or tissue or cultured cell lines.


The Research Specialist will also independently design and carry out experiments, interpret results, contribute to the planning and writing of any relevant publications.


Specific Duties & Responsibilities:


  • Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include: q/PCR, Western blot, and tissue culture and histochemistry/microscopy experiments.
  • Performs PCR genotyping and breeding of mice to maintain and expand mouse colonies for laboratory experiments on immune cell function. Works with graduate students, postdoctoral fellows and other personnel to maintain, expand mouse lines and generate new mutant mouse lines.
  • Assists with maintenance of mouse colony and participates in characterizing the molecular, cellular phenotypes of mice in the mouse model of allergic lung inflammation. Individual will manage mouse databases, weaning, tagging, sample collection, and PCR genotyping.
  • May be involved with recruitment and screening of Study Participants for IRB-approved blood draw or lung macrophage protocol. Individual will isolate immune cells from human blood, culture cells and analyze cells by FACS.
  • Monitors inventory levels, orders materials and supplies including lab consumables, glassware, and animals in accordance with established policies and procedures, counts orders upon receipt.
  • Possibly some training of undergraduates or graduate students in a few tasks.


Minimum Qualifications:

Bachelor's degree in biology, chemistry or related field.  Two (2) years laboratory experience performing relevant techniques.  Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.


Preferred Qualifications:

Master’s degree preferred.


JHU Equivalency Formula:  30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience.    For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum experience required for the respective job.


Additional Knowledge, Skills, and Abilities:

 Previous laboratory experience necessary, particularly in multi-color flow cytometry. Experience in mouse breeding and/or animal husbandry preferred. Excellent organization and communication skills are essential for working within our laboratory group.


Physical Requirements:


Sitting, standing and walking for extended period. Reaching by extending hand(s) or arm(s) in any direction.  Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s).  Ability to move standard equipment through a hospital or clinical environment.  Able to move boxes of lab supplies, equipment (light lifting).




Classified Title: Research Specialist II   ​​​​​
Role/Level/Range: ACRP/03/MB 
Starting Salary Range: $38,920.00 - $53,520.00; Commensurate with experience
Employee Group: Full Time 
Schedule: Monday - Friday, 40 hours 
Exempt Status: Exempt  
Location: School of Medicine, East Baltimore Campus 
Department Name: Anesthesiology Research 
Personnel Area: School of Medicine


The successful candidate(s) for this position will be subject to a pre-employment background check.


If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.


The following additional provisions may apply depending on which campus you will work.  Your recruiter will advise accordingly.


During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.


The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.


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School of Medicine - East Baltimore Campus