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Requisition ID:  118392

Research Technologist

We are seeking a Research Technologist to work in the Coyne Lab in the School of Medicine. The Coyne Lab uses induced pluripotent stem cell (iPSC) derived neuron (iPSN) and postmortem human tissue models to understand the mechanistic basis and pathophysiologic consequences of impaired nuclear pore complex (NPC) and nuclear envelope surveillance and homeostasis in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).


The Research Technologist will assist with various projects in the lab and primarily be responsible for the maintenance and differentiation of iPSNs, processing of samples for western blot and qRT-PCR, and carrying out western blots and qRT-PCR experiments. The technology may also assist with immunostaining and confocal imaging as required. We are looking for a candidate with a basic understanding of techniques and a foundation in the underlying science. The individual will work independently to carry out daily tasks with oversight from the PI.


The Research Technologist will participate in planning experiments and evaluating/interpreting test results utilizing knowledge of research laboratory procedures and practices. The individual will use independent judgment in the selection, development, performance and evaluation of research methods and operation of appropriate equipment.


Specific Duties and Responsibilities 

  • Follow established procedures or protocols and utilize aseptic technique to perform laboratory experiments which include: iPSN culture (passaging and maintenance), qRT-PCR, western blotting, cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.
  • Use various software applications, such as Excel spreadsheets, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble and/or format data.
  • Participate in the organization and operation of the laboratory.
  • Maintain written records and electronic databases.
  • Prepare solutions and reagents following standard laboratory formulas and procedures.
  • Plan and execute a defined set of experiments following discussion with the principal investigator.
  • Use universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
  • Complete annual university biohazard/universal precaution safety training, as appropriate.
  • Monitor biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.


Minimum Qualifications
  • Bachelor's Degree in Biology, Chemistry or related field.
  • Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.


Preferred Qualifications
  • Some knowledge of, or experience with, aseptic mammalian cell culturing.
  • Basic understanding of, or experience with, western blotting, qRT-PCR, or immunostaining.

 

 

Classified Title: Research Technologist  
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25 HRLY ($36,000 targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: M-F/40 
FLSA Status: Non-Exempt 
Location: School of Medicine Campus  
Department name: Neurodegenerative and Injury Program  
Personnel area: School of Medicine 

 

 

Total Rewards
The referenced base salary range represents the low and high end of Johns Hopkins University’s salary range for this position. Not all candidates will be eligible for the upper end of the salary range. Exact salary will ultimately depend on multiple factors, which may include the successful candidate's geographic location, skills, work experience, market conditions, education/training and other qualifications. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/.

Education and Experience Equivalency
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

Applicants Completing Studies
Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

Background Checks
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

Diversity and Inclusion
The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.

Equal Opportunity Employer
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

EEO is the Law
https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf

Accommodation Information
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit https://accessibility.jhu.edu/.

Vaccine Requirements
Johns Hopkins University strongly encourages, but no longer requires, at least one dose of the COVID-19 vaccine. The COVID-19 vaccine does not apply to positions located in the State of Florida. We still require all faculty, staff, and students to receive the seasonal flu vaccine. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. This change does not apply to the School of Medicine (SOM). SOM hires must be fully vaccinated with an FDA COVID-19 vaccination and provide proof of vaccination status. For additional information, applicants for SOM positions should visit https://www.hopkinsmedicine.org/coronavirus/covid-19-vaccine/ and all other JHU applicants should visit https://covidinfo.jhu.edu/health-safety/covid-vaccination-information/.

The following additional provisions may apply, depending upon campus. Your recruiter will advise accordingly.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
School of Medicine - East Baltimore Campus

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