Research Technologist

Welcome to the Watanabe Lab at Johns Hopkins University – A vibrant and pioneering hub of neurobiology research, where we delve into the intricate world of synaptic transmission. As you consider the possibility of joining our team as a Research Technologist, allow us to paint a vivid picture of who we are:

• Our central focus is synaptic transmission, unraveling the mechanisms that underlie communication between neurons.
• We explore the pathways that sustain neurotransmission and how other cell types and states influence this.
• We are a growing and evolving lab with members ranging from undergraduates, research technologists, graduate students, post-docs, and research associates.
• We employ a variety of techniques from electron microscopy, super resolution and live imaging, and electrophysiology.

Our Lab Culture

• Beyond traditional academic hierarchies, we cultivate an inclusive and collaborative culture that celebrates diversity.
• We believe in fostering an environment where every voice is heard, regardless of background or experience.
• We embrace a flat organizational structure, where ideas are valued based on their merit, and collaborative efforts drive us forward.
• Your unique perspective will enrich our collective understanding, and your contributions will be celebrated as essential pieces of our research puzzle.
• We value innovation and adaptability in our research methods, ideas, and interactions.

Your Role as a Research Technologist in our Lab

• Our research extends into the dynamic role of microglia, the brain's immune cells, in shaping and modulating synaptic connections.
• As a Research Technologist on this project, you will be at the heart of discovery, contributing to the understanding of the fundamental functions of microglia at the synapse.
• This role serves as a bridge between the minor and significant purposes of our lab, as you'll contribute both to the day-to-day operations and the grand narrative of our science.
• You will have the opportunity to be an active participant in our lab, conducting experiments, collecting and analyzing data, and translating observations into meaningful insights.
• You will collaborate closely with a diverse team of scientists, from enthusiastic students to established post-docs and research associates.
• Your skill set will be honed, providing you with opportunities to engage in discussions and foster your professional development.

Specific Duties & Responsibilities

• Collaborate with senior researchers to design and plan experiments.
• Independently conduct experiments while adhering to established protocols and safety guidelines.
• Producing lentiviruses for gene manipulation and DNA cloning for vector construction.
• Isolate and culture microglia from brain tissue.
• Employ imaging techniques (immunohistochemistry, confocal, and electron microscopy) to visualize neural structures and microglia in both cultured cells and tissue.
• Record and analyze data, utilizing software tools for statistical analysis and visualization.
• Organize, prepare, and manage laboratory reagents, ensuring equipment and materials are ready for experiments.
• Contribute to maintaining a clean and safe working environment, following proper waste disposal procedures.
• Keep detailed and accurate records of experimental procedures, observations, and outcomes.
• Assist in preparing research findings for presentations at lab meetings, conferences, and publications.
• Collaborate effectively with fellow researchers, sharing insights, troubleshooting, and contributing to a dynamic scientific dialogue.
• Communicate experimental results clearly in discussions, presentations, and written reports.
• Embrace the lab's inclusive culture by fostering a collaborative and respectful environment.

Knowledge, Skills, and Abilities

• Experience in cell culture methods, including primary cultures.
• Knowledge of immunostaining and confocal microscopy.
• Familiarity with molecular biology techniques such as PCR, cloning, and DNA Maxi-preps.
• Ability to troubleshoot experimental protocols and identify potential technical issues.
• Strong analytical skills to interpret complex data and contribute to scientific discussions.
• Detail-oriented approach for precise documentation of experiments, protocols, and results.
• Excellent time management skills to handle multiple tasks, prioritize, and meet project deadlines.
• Effective interpersonal skills to collaborate with a diverse team and contribute to a harmonious work environment.
• Adaptable mindset to embrace new challenges and pivot research directions as needed.

• Bachelor's Degree in cell biology, molecular biology, chemistry, or related field.
• Some related post-undergraduate work experience preferred.
• Some related laboratory work experience preferred.
• Additional education may substitute for required experience, and additional related experience may substitute for required education beyond a High School Diploma/Graduation Equivalent, to the extent permitted by the JHU Equivalency formula.

• Training in biosafety protocols and adeptness in handling viral vectors within laboratory environments.
• Experience in animal handling and proper care of research animals.
• Research background in the realm of neurobiology.
• Experience in electron microscopy techniques.

Classified Title: Research Technologist  
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25/HRLY ($42,000.00 Annually/Targeted; Commensurate with experience) 
Employee group: Full Time 
Schedule: Monday-Friday, 8:30 AM - 5:00 PM 
FLSA Status: Non-Exempt 
Location: School of Medicine - East Baltimore Campus 
Department name: SOM, Cell Biology 
Personnel area: School of Medicine