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Job Req ID:  18853

Research Technologist


General Summary/Purpose:

The research in the Center for Neurogastroenterology is focused on the diseases that cause intestinal dysmotility and is hiring a Laboratory Research Technologist. Under the direct supervision of the Faculty and other senior staff in the Center of Neurogastroenterology, the laboratory technologist will be responsible for carrying out tissue dissociations, library preparations, 10X Genomics Chromium based assays and other relevant protocols for single cell RNA sequencing of various intestinal cells and neurons that innervate various parts of the gastrointestinal tract.

The candidate will assist the research studies and will be responsible for processing samples from rodent and human specimens for their molecular analyses at a single cell level using 10X Genomics Chromium based platform and for subsequent single cell RNA sequencing.


Specific Duties & Responsibilities:
He or She will perform a variety of research laboratory techniques with primary focus on the cell biology related to the study of gastrointestinal motility and pain disorders. Techniques to be performed include, but are not limited to: tissue harvesting, tissue dissociation, RNA and DNA estimation, protein estimation, 10X Genomics Chromium platform based assays, cDNA library preparations. Various lab-based duties that he or she will perform include:

  • Preparation of buffers and reagents required for tissue isolation.
  • Performing tissue harvests from various rodent and human samples.
  • Help standardize and carry out single cell isolation from harvested tissues.
  • Estimate cell viability, nucleic acid and protein concentrations.
  • Help run assays for cDNA library preps using conventional and 10X Genomics Chromium platform.
  • Performs and evaluates research methods under supervision.
  • Documenting and reporting all experimental results
  • Creating and maintaining stocks of all buffers, reagents, media, and antibodies.
  • Operates appropriate equipment.
  • Uses sterile techniques to avoid contaminating lab experiments. Be knowledgeable of and employ good tissue culture techniques.
  • Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.


Minimum Qualifications (Mandatory):

  • Bachelor’s degree in biology required. 
  • Masters degree preferred.
  • This work requires precision and attention to detail. Individual should have strong communication and interpersonal skills.


Special Knowledge, Skills, and Abilities: 

  • Must have the ability to read and interpret direction, both oral and written, organize and prioritize work and follow through on assignments. 
  • Must be able to work successfully as part of a team in a dynamic laboratory.

Any Specific Physical Requirements for the Job:

  • Sitting in a normal seated position in office setting.
  • Standing and/or walking for extended periods of time.
  • Lifting and/or assisting patients during evaluations within crowded clinical environment.
  • Reaching by extending hand(s) or arm(s) in any direction. 
  • Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard. 
  • Communication skills using the spoken word. 
  • Ability to see within normal parameters. 
  • Ability to hear within normal range. 
  • Ability to move about. 


Classified Title: Research Technologist 
Working Title: Research Technologist 
Role/Level/Range: ACRO40/E/02/CC 
Starting Salary Range: $14.14 - $19.44 per hour
Employee group: Full Time 
Schedule: 9-5 Monday-Friday 
Exempt Status: Non-Exempt  
Location: 04-MD:School of Medicine Campus 
Department name: 10002808-SOM DOM Gastroenterology 
Personnel area: School of Medicine


The successful candidate(s) for this position will be subject to a pre-employment background check.


If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at For TTY users, call via Maryland Relay or dial 711.


The following additional provisions may apply depending on which campus you will work.  Your recruiter will advise accordingly.


During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.


The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.


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