Share this Job
Job Req ID:  107177

Research Specialist II

The Trapecar Lab at the John Hopkins All Children’s Hospital and the Institute for Fundamental Biomedical Research in St. Petersburg, Florida, is seeking a Research Specialist II who will be responsible for independent scientific and administrative activities in our laboratory. We are looking for a new team member that is highly motivated, energetic, and independent, with an interest in modeling multiorgan human physiology and the application of such advanced in vitro models to gain a better understanding of the fundamental origins of immunometabolic diseases. The individual requires a good understanding of the overall objectives and interests of our research, as outlined on the lab’s website -

Specific Duties & Responsibilities

  • Design and perform experiments following established procedures, including whole blood/tissue processing, ELISA, Microscopy, DNA and RNA extractions, PCR, sequencing, biomarker, and cytokine multiplex assays.
  • Establish and maintain new cell cultures, including human organoids and iPS cells and make protocol modifications as needed.
  • Operate laboratory equipment such as flow cytometer, 3D bioprinter, bio-safety cabinet, centrifuge, incubator, ultra-low temperature freezer, LN2 freezer, multi-mode plate reader, light microscope, thermocycler, and others.
  • Keep detailed records of all the testing and help analyze the resulting data.
  • Disseminate study information directly to colleagues and collaborators
  • Participate in the writing of papers and presentations for meetings/conferences.
  • Assist with training of new colleagues on specific lab procedures.
  • Use databases to maintain sample inventories and related data.
  • Operate lab computers for data entry and maintain accurate data records.
  • Assist with general lab maintenance, lab safety, manage and make purchases of basic laboratory supplies and specific project necessities and maintain necessary lab inventory.
  • Perform necessary administrative tasks such as but not limited to grant progress reports.

Special Knowledge, Skills, & Abilities

  • Excellent mammalian cell or cell line culture skills.
  • Conversant with various analytical methodologies including but not limited to flow cytometry, ELISA/Luminex, and microscopy. If not conversant, the expectation is for the candidate to get acquainted with the technology and become independent within six months of joining.
  • Knowledge of human immune cell enrichment and manipulation.
  • Knowledge of cell-based binding assays, surface antigen and intracellular staining methodology.
  • Excellent record keeping, organizational, written and verbal skills
  • Familiar with routine immune functional assays for human immune cell populations.
  • Able to set up assays and conduct technical troubleshooting analysis.
  • Able to analyze data and provide a critical interpretation of results.
  • Ability to handle multiple priorities in a fast-paced environment.
  • Strong interpersonal skills for interfacing with other functional groups.

Physical Requirements:

  • Employee may remain in a stationary position (sitting or standing) for up to 40% of the time.
  • The employee is required to operate small laboratory tools that require manual dexterity.
  • Prolonged vision requirements including viewing computer screens, paper reports/documents, charts, and results.
  • Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope)
  • Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed.
  • Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas.
  • Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area.
  • Working in laboratory where there may be discomfort due to odors, noise, temperature fluctuations, and working around lab equipment.
  • Research areas may include animal, human, human products, DNA, and radiation protocols.
  • Use of personal protective equipment may be advised or required.
  • May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory).
  • May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes). In some areas work space is limited.

Minimum Qualifications
  • Bachelor's Degree in Biology, Chemistry or related field.
  • Two years laboratory experience performing relevant techniques.
  • Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
  • Additional education may substitute for the required experience.



Classified Title: Research Specialist II 
Working Title: Research Specialist II   
Role/Level/Range: ACRP/03/MB  
Starting Salary Range: $40,100-$55,140-$70,180 Annually (Commensurate with experience) 
Employee group: Full Time 
Schedule: 8 hours/day. Work on weekends and prolonged hours might occasionally be required. 
Exempt Status: Exempt 
Location: All Children's Hospital - Florida 
Department name: 60002426-All Childrens 
Personnel area: School of Medicine 


Total Rewards
The referenced salary range is based on Johns Hopkins University’s good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here:


Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate’s conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.


The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.


Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.


EEO is the Law
Learn more:


Accommodation Information

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit


Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit and all other JHU applicants should visit


The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.


Note: Job Postings are updated daily and remain online until filled.



All Children's Hospital - Florida